26 August 2015

From sensory processing to functional hyperaemia


Members : Ali-Kémal AYDIN (PhD Student), Emmanuelle CHAIGNEAU (Research Engineer), Yannick GOULAM HOUSSEN (Research Engineer), Florent JEAN (Administrator), Manon OMNES (Technician) , Morgane ROCHE (PhD Student), Ravi RUNGTA (Post Doc)

My team aims at understanding how interactions between neurons and non-neuronal cells modulate brain activity and how this modulation contributes to the signals used in functional imaging of the human brain.
This imposes to work at all spatial and temporal scales and to understand deeply the relationship between the activity of a given neuronal network and the nature of the imaging signal used to detect it.
Using cellular approaches (electrophysiology, and two-photon laser scanning microscopy), we investigate the functional responses of the glomerulus network, i.e. the responses to odor of neuron, glial cells and vascular cells in (pericytes and smooth muscle cells), and we correlate these responses with metabolic (oxygen, phosphorescence lifetime imaging) and macroscopic vascular responses measured with fast ultrasound imaging and BOLD MRI (through a collaboration).



Brown R, Benveniste H, Black SE, Charpak S, Dichgans M, Joutel A, Nedergaard M, Smith KJ, Zlokovic BV, Wardlaw JM. “Understanding the role of the perivascular space in cerebral small vessel disease.” Cardiovasc Res. 1;114(11) (2018)

Rungta RL, Chaigneau E, Osmanski BF, Charpak S. “Vascular Compartmentalization of Functional Hyperemia from the Synapse to the Pia.” Neuron. 25;99(2):362-375 (2018)

Rungta, RL, Osmanski, B, Boido, D. Tanter, M, Charpak, S. “Light controls cerebral blood flow in naive animals.” Nat Commununications 8, 14191. (2017).

Rungta RL, Charpak S “Astrocyte endfeet march to the beat of different vessels.” Nat Neurosci 19:1539-1541. (2016)

Lyons D G, Parpaleix A, Roche M, Charpak S  “Mapping oxygen concentration in the awake mouse brain”. eLife 2016;5:e12024. (2016)

Collot M, Wilms CD, Bentkhayet A, Marcaggi P, Couchman K, Charpak S, Dieudonne S, Hausser M, Feltz A, Mallet JM “CaRuby-Nano: a novel high affinity calcium probe for dual color imaging.” eLife 2015;4:e05808. (2015)

Najac M, Sanz Diez A, Kumar A, Benito N, Charpak S, De Saint Jan D  “Intraglomerular lateral inhibition promotes spike timing variability in prinicipal neruons of the olfactory bulb.” J Neurosci 35:4319-31. (2015)

Otsu Y, Couchman K, Lyons DG, Collot M, Agarwal A, Mallet JM, Pfrieger FW, Bergles DE, Charpak S “Calcium dynamics in astrocyte processes during neurovascular coupling.” Nat Neurosci 18:210-218. (2015)

Ducros M, Goulam Houssen Y, Bradley J, de Sars V, Charpak S “Encoded multisite two-photon microscopy.” Proc Natl Acad Sci USA, vol. 110 13138-13143. (2013)

Parpaleix A, Goulam Houssen Y, Charpak S, “Imaging local neuronal activity by monitoring PO(2) transients in capillaries.” Nat Med. vol.19 241-246. (2013)

Charpak S, Stefanovic B , “Shedding light on the BOLD fMRI response.” Nat Methods. vol.9. 547-549. (2012)

J. Lecoq, A. Parpaleix, E. Roussakis, M. Ducros, Y. Houssen, S. Vinogradov, S. Charpak “Simultaneous two-photon imaging of oxygen and blood flow in deep cerebral vessels.” Nature Medicine. vol.17:893-898. (2011)

N. Jukovskaya, P. Tiret, J. Lecoq, S. Charpak “What Does Local Functional Hyperemia Tell about Local Neuronal Activation?.” J Neurosci. vol.31. 5. Feb. 1579 –1582. (2011)

M. Ducros M, Van’t HM, Evrard A, Seebacher C, Schmidt EM, Charpak S, Oheim M, “Efficient large core fiber-based detection for multi-channel two-photon fluorescence microscopy and spectral unmixing.” J. Neurosci. Meth. vol.198:172-180. (2011)

D. Attwell, A. Buchan, S. Charpak, M. Lauritzen, B. MacVicar, E. Newman “Glial and neuronal control of brain blood flow”. Nature. vol.468. Nov. 232–243. (2010)

P. Tiret, E. Chaigneau, J. Lecoq, S. Charpak, “Two-photon imaging of capillary blood flow in olfactory bulb glomeruli.” Methods Mol Biol. 489:81-91. (2009)

J. Lecoq, P. Tiret, S. Charpak, “Peripheral adaptation codes for high odor concentration in glomeruli.” Journal of Neuroscience. vol.29. 10. Mar 11. 3067-72. (2009)

J. Lecoq, P. Tiret, M. Najac, G. Shepherd, C. Greer, S. Charpak,”Odor-evoked oxygen consumption by action potential and synaptic transmission in the olfactory bulb.” J. Neurosci. vol.29. 5. Feb 4. 1424-33. (2009)

M. Ducros, L. Moreaux, J. Bradley, O. Griesbeck, S. Charpak, “Spectral Unmixing: Analysis of Performance in the Olfactory Bulb in vivo.” PLoS ONE. vol.4. 2. Feb 9. e4418. (2009)

D. De Saint Jan, D. Hirnet, G. Westbrook, S. Charpak, “External tufted cells drive the output of olfactory bulb glomeruli.” J. Neurosci.. vol.29. 7. Feb 18. 2043-52. (2009)

E. Chaigneau, P. Tiret, J. Lecoq, M. Ducros, T. Knöpfel, S. Charpak, “The Relationship between Blood Flow and Neuronal Activity in the Rodent Olfactory Bulb.” Journal of Neuroscience. vol.27. June. 6452-6460. (2007)