26 August 2015

Neuron-glia interactions

PI : Etienne AUDINAT

Members : Coralie-Anne MOSSER, Ljiljana NIKOLIC, Paola NOBILIManon OMNES

Microglial cells (green) in the whisker-related barrel field of the mouse somatosensory cortex surround clusters of thalamo-cortical axons (i.e. the barrel centers, red) at early stages of the postnatal development (adapted from Hoshiko et al., 2012).

Microglial cells (green) in the whisker-related barrel field of the mouse somatosensory cortex surround clusters of thalamo-cortical axons (i.e. the barrel centers, red) at early stages of the postnatal development (adapted from Hoshiko et al., 2012).

Glial cells are not only supportive cells in the central nervous system (CNS) but are also engaged with neurons in bi-directional interactions that contribute actively to the modulation of neuronal network activities. These interactions are based on the expression by glial cells of a large repertoire of receptors for neurotransmitters and on their ability to release neuro-active compounds (gliotransmitters).
Our research focuses on the interactions between neurons, astrocytes and microglia in the CNS of rodents and relies on a multidisciplinary approach based on electrophysiology, calcium imaging, optogenetic, molecular biology and histochemical techniques that can be combined on single cells of acute brain slices. Two main research programs structure the current activity of the team.
• The roles of astrocytes and microglia in the epileptic hippocampus
• The roles of microglia during the postnatal development of the neocortex

 

Selected publications:

Mosser C-A, Baptista S, Arnoux I, Audinat E (2017) Microglia in the CNS development: Shaping the brain for the future. Progress in Neurobiology, doi: 10.1016/j.pneurobio.2017.01.002 (Review)

Rassendren F, Audinat E (2016) Purinergic signaling in epilepsy. Journal of Neuroscience Research 94(9):781-93 (Review)

Arnoux I, Audinat E (2015) Fractalkine signaling and microglia functions in the developing brain. Neural Plasticity, special issue “Glial Plasticity”, doi 10.1155/2015/689404 (Review)

Arnoux I, Hoshiko M, Sanz Diez A, Audinat E (2014) Paradoxical effects of minocycline in the developing mouse somatosensory cortex. Glia 62(3): 399-410

Audinat E, Arnoux I (2014) La microglie: des cellules immunitaires qui sculptent et contrôlent les synapses neuronales. Médecine/Sciences 30 : 153-1559 (Review)

Carrillo-de Sauvage MA, Pasco M, Arnoux I, Maatouk L, Sanz Diez A, Delahaye M, Herrero MT, Newman TA, Calvo CF, Audinat E, Tronche F, Vyas S (2013) Potent and multiple regulatory and anti-inflammatory actions of microglial glucocorticoid receptors during inflammation. Cell Death and Differentiation (20): 1546-1557

Arnoux I, Hoshiko M, Avignone E, Mandavy L, Yamamoto M, Audinat E (2013) Adaptive phenotype of microglial cells during the normal postnatal of the somatosensory “barrel” cortex. Glia 61(10): 1582-94

Ulmann L, Levavasseur F, Avignone E, Hibec H, Audinat E*, Rassendren f* (2013) Involvement of P2X4 receptors in hippocampal microglia activation after status epilepticus. Glia 61(8): 1306-19

Hoshiko M, Arnoux I, Avignone E, Yamamoto N, Audinat E (2012) Deficiency of the microglial receptor CX3CR1 impairs postnatal functional development of thalamo-cortical synapses in the barrel cortex. The Journal of Neuroscience 32:15106-15111

Le Meur K, Mendizabal-Zubiaga J, Grandes P, Audinat E (2012) GABA release by hippocampal astrocytes. Frontiers in Computational Neuroscience 6:59. doi: 10.3389/fncom.2012.00059

Avignone E, Ulmann L, Levavasseur F, Rassendren f, Audinat E (2008) Status epilepticus induces a particular microglial activation state characterized by enhanced purinergic signaling. The Journal of Neuroscience 28: 9133-9144

Angulo MC, Le Meur K, Kozlov A, Charpak S, Audinat E (2008) GABA: a forgotten gliotransmitter. Progress in Neurobiology 86:297-303 (Review)